Coding

Part:BBa_M11099:Design

Designed by: ryan dunoskovic   Group: UtahState BE5930 - S09 UtahState BE5930 - S10   (2010-04-22)


porcine coagulation factor VIII


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 920
    Illegal BglII site found at 1680
    Illegal BglII site found at 4037
    Illegal BamHI site found at 720
    Illegal BamHI site found at 1869
    Illegal BamHI site found at 5219
    Illegal BamHI site found at 5598
    Illegal BamHI site found at 6323
    Illegal XhoI site found at 1656
    Illegal XhoI site found at 4761
    Illegal XhoI site found at 6364
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 779
    Illegal BsaI site found at 2113
    Illegal BsaI site found at 6011
    Illegal BsaI.rc site found at 4258
    Illegal SapI site found at 169


Design Notes

contained RE sites EcoRI (3), PstI (5), and XbaI (1) which had to be modified for standardization.


Source

complete cDNA sequence found in NCBI database.

References